Purification and Characterization of a Novel Intracellular Sucrase Enzyme of Leishmania donovani Promastigotes

Figure 5

Effect of metal ion on the intracellular sucrase enzyme activity. The purified enzyme was preincubated with metal ions for 10 min at room temperature and the activity assay was performed in 50 mM sodium acetate buffer at pH 6.0. The enzyme activity in absence of metal ions was considered to be 100% and compared the relative activity of the enzyme in presence of different metal ions. The value presented corresponds to the mean value of three replicates.