Protective Effect of Edaravone against Carbon Monoxide Induced Apoptosis in Rat Primary Cultured Astrocytes

Figure 4

The results of ROS measurement, real-time PCR analysis, and Western blot analysis. (a) Cellular ROS level of each group was measured using FCM with DCFH-DA kit. (A) Control group, (B) CO group, (C) 0.1 μM Eda group, (D) 1.0 μM Eda group, (E) 10 μM Eda group, and (F) 50 μM Eda group. (b) The relative mRNA expressions of Bcl-2, Bax, and cleaved caspase-3 were estimated using real-time PCR analysis. Compared with CO group: , . (c) The protein expressions of Bcl-2, Bax, and caspase-3 were estimated using Western blot analysis. Compared with CO group: , . For each method, three biological replicates were prepared for each sample.

(a)

(b)

(c)