Aqueous Extract of Leaves and Flowers of Acmella caulirhiza Reduces the Proliferation of Cancer Cells by Underexpressing Some Genes and Activating Caspase-3

Table 2

Primers of the genes used (Sigma-Aldrich DNA oligos template).

Name	Code	Scale	Forward sequence (5′ ⟶ 3′)	Reverse sequence (5′ ⟶ 3′)
TNF-α	STD	0.025	GGT​GCC​TAT​GTC​TCA​GCC​TCT​T	GCC​ATA​GAA​CTG​ATG​AGA​GGG​AG
TGFb1	STD	0.025	TGA​TAC​GCC​TGA​GTG​GCT​GTC​T	CAC​AAG​AGC​AGT​GAG​CGC​TGA​A
IL-6	STD	0.025	TAC​CAC​TTC​ACA​AGT​CGG​AGG​C	CTG​CAA​GTG​CAT​CAT​CGT​TGT​TC
IL-1β	STD	0.025	CTT​GGG​CCT​CAA​AGG​AAA​GAA	AAG​ACA​AAC​CGT​TTT​TCC​ATC​TTC
IL-10	STD	0.025	CGG​GAA​GAC​AAT​AAC​TGC​ACC​C	CGG​TTA​GCA​GTA​TGT​TGT​CCA​GC
Bcl-2	STD	0.025	ATG​CCT​TTG​TGG​AAC​TAT​ATG​GC	GGT​ATG​CAC​CCA​GAG​TGA​TGC
Bcl-6	STD	0.025	CCT​GAG​GGA​AGG​CAA​TAT​CA	GTT​TAA​GTG​CAG​GGG​CCA​TT
HPRT-1	STD	0.025	GCA​GAC​TTT​GCT​TTC​CTT​GG	CGA​GAG​GTC​CTT​TTC​ACC​AG
GAPDH	STD	0.025	GCA​AGA​GAG​AGG​CCC​TCA​G	TGT​GAG​GGA​GAT​GCT​CAG​TG
IGF-1	STD	0.025	GTG​TGG​ACC​GAG​GGG​CTT​TTA​CT	GCT​TCA​GTG​GGG​CAC​AGT​ACA​TC
E2F1	STD	0.025	GAT​GGT​GGG​GCT​GAT​ATT​TG	CAG​CGA​GGT​ACT​GAT​GGT​CA
E2F2	STD	0.025	ACA​ACA​TCC​AGT​GGG​TAG​GC	GGG​AGC​AAC​TCT​GAA​TGA​GC
VEGF	STD	0.025	CAC​AGC​AGA​TGT​GAA​TGC​AG	TTT​ACA​CGT​CTG​CGG​ATC​TT
β-Actin	STD	0.025	ACG​ACG​TGG​CAG​CTC​TCG​TTG​TGG	GGT​GCT​TCG​GTC​AGC​AGC​ACG​GA
PDGF	STD	0.025	TAG​CGC​GGA​ACC​TCA​GAG​AGA	TGG​GAG​GTC​CCC​ATA​GCT​CC

E2F: transcription factors of the E2F family; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HPRT-1: hypoxanthine phosphoribosyltransferase-1 enzyme; IGF-1: insulin-like growth factor 1; Bcl: B cell lymphoma; IL: interleukin; PDGF: platelet-derived growth factor; TGFβ: transforming growth factor β; TNF-α: tumour necrosis factor-α; VEGF: vascular endothelial growth factor.