Mass Spectrometry of Putrescine, Spermidine, and Spermine Covalently Attached to <i>Francisella tularensis</i> Universal Stress Protein and Bovine Albumin

<div>Structures of the polyamines putrescine, spermidine, and spermine. The monoisotopic molecular weights were calculated from their molecular formulas using Exact Mass Calculator, Single Isotope version from Scientific Instrument Services by Adaptus Solutions. Covalent binding of a polyamine to glutamine by transglutaminase causes the loss of NH<sub>3</sub> (17 Da), whereas covalent binding of a polyamine to glutamate and aspartate via modification by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide causes the loss of H<sub>2</sub>O (18 Da).</div>

Biochemistry Research International

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Figure 1

Figure 1: Mass Spectrometry of Putrescine, Spermidine, and Spermine Covalently Attached to <i>Francisella tularensis</i> Universal Stress Protein and Bovine Albumin 

Figure 1 | Mass Spectrometry of Putrescine, Spermidine, and Spermine Covalently Attached to Francisella tularensis Universal Stress Protein and Bovine Albumin 