Research Article

Mass Spectrometry of Putrescine, Spermidine, and Spermine Covalently Attached to Francisella tularensis Universal Stress Protein and Bovine Albumin

Figure 2

Formation of a glutamate-putrescine adduct catalyzed by EDC and formation of a glutamine-putrescine adduct catalyzed by transglutaminase. EDC catalyzes the covalent attachment of putrescine to the acid side chains of glutamic (E) or aspartic acid (D) to make an isopeptide bond. Transglutaminase catalyzes the covalent attachment of putrescine to the amide side chain of glutamine (Q) to make an isopeptide bond.