Biochemistry Research International

Research Article

Mass Spectrometry of Putrescine, Spermidine, and Spermine Covalently Attached to Francisella tularensis Universal Stress Protein and Bovine Albumin

Table 2

Polyamine modified peptides in bovine serum albumin that are labeled by the action of EDC on glutamate and aspartate.
Polyaminated BSA peptideLabeled residuePolyamineSignature ions DaNeutral loss, DaDiscriminant score
NELTEFAKE69Spermidine 714.27
NELTEFAKE69Putrescine155, 200 5.31
VNELTEFAKE69Putrescine155, 200 5.52
LVNELTEFE69Putrescine155 6.35
LVNELTEFAKE69Putrescine155, 200 5.16
LVNELTEFAKE72Putrescine129, 155, 200 3.89
SLHTLFGDELCKE97Putrescine129 5.95
NECFLSKKE124Spermine195, 269 3.86
LKPDPNTLCDEFKE149Spermidine  6.41
YNGVFQECCQAEDKE195Putrescine  4.59
YYANKYNGVFQECCQAEDKE195Putrescine  6.34
HPYFYAPELLYYANKYNGVFQECCQAEDKE195Putrescine  7.20
AEFVEVTKLVTDLTKE250Putrescine155, 200 6.68
LSQKFPKAEFVEVTKE250Putrescine  6.03
LVTDLTKVHKECCHGDLLECADDRE275Putrescine  7.34
DAIPENLPPLTADFAEDKE323Putrescine  6.58
DAIPENLPPLTADFAEDKDVCKE334Putrescine  7.64
DAIPENLPPLTADFAEDKDVCKE334Spermidine186 6.61
PENLPPLTADFAEDKDVCKE334Putrescine  8.15
NYQEAKDAFLGSFLYEYSRE344Putrescine  6.89
DVCKNYQEAKDAFLGSFLYEYSRE344Putrescine  8.55
LGSFLYEYE356Putrescine129, 155, 200 3.89
DAFLGSFLYEYE356Putrescine155, 200 5.30
DAFLGSFLYEYE356Spermine269744.30
DAFLGSFLYEYE356Spermidine  4.12
DAFLGSFLYEYSRE356Putrescine  6.64
DAFLGSFLYEYSRE356Spermidine186714.98
HPEYAVSVLLRE363Putrescine129 5.88
HLVDEPQNLIKE406Putrescine155 4.67
LGEYGFQNALIVRE423Putrescine155 6.40
LGEYGFQNALIVRE423Spermine195, 269 4.97
LGEYGFQNALIVRE423Spermine195, 269 4.86
PTLVEVSRE448Putrescine129 5.74
PTLVEVSRE448Spermidine 713.81
TPTLVEVSRE448Putrescine129 6.51
TPTLVEVSRE448Spermidine 714.36
STPTLVEVSRE448Putrescine129 5.67
VSTPTLVEVSRE448Putrescine129 6.73
VPQVSTPTLVEVSE448Spermine269 4.67
VPQVSTPTLVEVSRE448Putrescine  7.75
VPQVSTPTLVEVSRE448Spermidine 716.88
KVPQVSTPTLVEVSRE448Putrescine  7.62
KVPQVSTPTLVEVE448Spermidine  4.85
YTRKVPQVSTPTLVEVSRE448Putrescine  7.20
TEDYLSLILNRE473Putrescine  6.52
TVMENGVAFVDKE512Spermine195, 269744.49
TVMENGVAFVDKE512Putrescine200 5.20
PDETYVPKE518Putrescine155 5.58
PDETYVPKE518Spermidine  3.54
RPCFSALTPDETYVPKE518Putrescine155 5.32
LFTFHADICTLPDTEKE543Putrescine  6.50
SLHTLFGDELCKD96Putrescine141 6.03
PNTLCDEFKD48Putrescine141 4.41
NLPPLTADFAEDKD331Putrescine141 6.58
PENLPPLTADFAEDKD331Putrescine141 7.39
DAIPENLPPLTADFAEDKD319Spermine300 6.13
DAIPENLPPLTADFD331Spermine272745.31
DAIPENLPPLTADFD331Putrescine  6.41
DAIPENLPPLTADFAEDKD335Spermidine  6.77
DAFLGSFLYEYD347Putrescine  4.09
DAFLGSFLYEYSRD347Spermine  7.01
DAFLGSFLYEYSRD347Spermine  4.96
DAFLGSFLYEYSRD347Spermine  7.10
DAFLGSFLYEYSRD347Spermine226 5.25
DAFLGSFLYEYSRD347Putrescine186 6.01
DAFLGSFLYEYSRD347Spermidine198 5.92
TEDYLSLILNRD474Spermine272 5.97
TEDYLSLILNRD474Putrescine  6.14
PCTEDYLSLILNRD474Putrescine  8.61
MPCTEDYLSLILNRD474Putrescine186 7.32
LFTFHADICTLPDTEKD535Putrescine  7.55
TFHADICTLPDTEKD541Putrescine  4.88
LFTFHADICTLPDTEKD541Spermidine  6.57
TVMENGVAFVDD579Putrescine186 5.11
TVMENGVAFVDKD579Spermine 743.89
TVMENGVAFVDKD579Spermine  4.25
TVMENGVAFVDKD579Spermine226744.47
TVMENGVAFVDKD579Putrescine186 5.46
TVMoxENGVAFVDKD579Spermine 743.70
TVMENGVAFVDKCCADDKD579Putrescine  5.69
Cysteines are carbamidomethylated, adding 57 Da to the mass of the peptide. A discriminant score greater than 0.0 is considered to be strong evidence for a correct match of the peptide sequence to the MS/MS spectrum [11].