Somatic Embryogenesis and Massive Shoot Regeneration from Immature Embryo Explants of Tef

Table 3

The effect of glutamine in the callus induction medium on callus formation, somatic embryogenesis, and shoot regeneration of intermediate-sized and large “Fesho” and “DZ-01-196” immature zygotic embryos. Values (within one row) followed by different superscript letters are significantly different from one another at 𝑃<0.05.

Genotype	Fesho					DZ-01-196
Glutamine [mM]	0	1	3	6	10	0	1	3	6	10
No. of cultivated embryos	75	75	75	75	75	60	60	62	69	60
% responding embryos*	46.7^c	28.0^d	57.3^b	94.7^a	90.7^a	66.7^a	38.3^b	96.8^a	100^a	100^a
% Embryos forming somatic embryos	14.7^d	10.7^d	56.0^b	84.0^a	40.0^c	35.0^c	25.0^d	71.0^b	94.2^a	23.3^d
% Embryos regenerating shoots	4.0^b	4.0^b	40.0^b	68.0^a	8.0^b	31.7^c	15.0^d	66.1^b	88.4^a	0^e

*Embryos showing callus formation and/or somatic embryogenesis.