Generation and Characterization of an scFv Directed against Site II of Rabies Glycoprotein

Table 1

cmd Primers used for PCR of VH and VL regions and SOE PCR for construction of scFv.

Variable light chain forward primers
LB1	GCCATGGCGGA(CT)ATCCAGCTGACTCAGCC
LB2	GCCATGGCGGA(CT)ATTGTTCTC(AT)CCCAGTC
LB3	GCCATGGCGGA(CT)ATTGTG(AC)T(AC)ACTCAGTC
LB4	GCCATGGCGGA(CT)ATTGTG(CT)T(AG)ACACAGTC
LB5	GCCATGGCGGA(CT)ATTGT(AG)ATGAC(AC)CAGTC
LB6	GCCATGGCGGA(CT)ATT(AC)AGAT(AG)A(AC)CCAGTC
LB7	GCCATGGCGGA(CT)ATTCAGATGA(CT)(AGT)CAGTC
LB8	GCCATGGCGGA(CT)AT(CT)CAGATGACACAGAC
LB9	GCCATGGCGGA(CT)ATTGTTCTCA(AT)CCAGTC
LB10	GCCATGGCGGA(CT)ATTG(AT)GCT(GC)ACCCAATC
LB11	GCCATGGCGGA(CT)ATT(GC)T(AG)ATGACCCA(AG)TC
LB12	GCCATGGCGGA(CT)(AG)TT(GT)TGATGACCCA(AG)AC
LB13	GCCATGGCGGA(CT)ATTGTGATGAC(GCT)CAG(GT)C
LB14	GCCATGGCGGA(CT)ATTGTGATAAC(CT)CAGGA
LB15	GCCATGGCGGA(CT)ATTGTGATGACCCAG(AT)T
LB16	GCCATGGCGGA(CT)ATTGTGATGACACAACC
LB17	GCCATGGCGGA(CT)ATTTTGCTGACTCAGTC
Variable light chain reverse primers
LF1	GGAGCCGCCGCCGCCAGAACCACCACCACCAGAACCACCACCACCACGTTTGATTTCCAGCTTGG
LF2	GGAGCCGCCGCCGCCAGAACCACCACCACCAGAACCACCACCACCACGTTTTATTTCCAGCTTGG
LF4	GGAGCCGCCGCCGCCAGAACCACCACCACCAGAACCACCACCACCACGTTTTATTTCCAACTTTG
LF5	GGAGCCGCCGCCGCCAGAACCACCACCACCAGAACCACCACCACCACGTTTCAGCTCCAGCTTGG
Variable heavy chain forward primers
HB1	GGCGGCGGCGGCTCCGGTGGTGGTGA(GT)GT(AG)(AC)AGCTTCAGGAGTC
HB2	GGCGGCGGCGGCTCCGGTGGTGGTGAGGT(GCT)CAGCT(GCT)CAGCAGTC
HB3	GGCGGCGGCGGCTCCGGTGGTGGTCAGGTGCAGCTGAAG(GC)A(GC)TC
HB4	GGCGGCGGCGGCTCCGGTGGTGGTGAGGTCCA(AG)CTGCAACA(AG)TC
HB5	GGCGGCGGCGGCTCCGGTGGTGGTCAGGT(CT)CAGCT(GCT)CAGCA(AG)TC
HB6	GGCGGCGGCGGCTCCGGTGGTGGTCAGGT(CT)CA(AG)CTGCAGCAGTC
HB7	GGCGGCGGCGGCTCCGGTGGTGGTCAGGTCCACGTGAAGCAGTC
HB8	GGCGGCGGCGGCTCCGGTGGTGGTGAGGTGAA(GC)(GC)TGGTGGAATC
HB9	GGCGGCGGCGGCTCCGGTGGTGGTGA(AGC)GTGA(AT)G(CT)TGGTGGAGTC
HB10	GGCGGCGGCGGCTCCGGTGGTGGTGAGGTGCAG(GC)(GT)GGTGGAGTC
HB11	GGCGGCGGCGGCTCCGGTGGTGGTGA(GT)GTGCA(AC)CTGGTGGAGTC
HB12	GGCGGCGGCGGCTCCGGTGGTGGTGAGGTGAAGCTGATGGA(AG)TC
HB13	GGCGGCGGCGGCTCCGGTGGTGGTGAGGTGCA(AG)CTTGTTGAGTC
HB14	GGCGGCGGCGGCTCCGGTGGTGGTGA(AG)GT(AG)AAGCTTCTCGAGTC
HB15	GGCGGCGGCGGCTCCGGTGGTGGTGAAGTGAA(AG)(GC)TTGAGGAGTC
HB16	GGCGGCGGCGGCTCCGGTGGTGGTCAGGTTACTCT(AG)AAAG(AT)GT(GC)TG
HB17	GGCGGCGGCGGCTCCGGTGGTGGTCAGGTCCAACT(AGC)CAGCA(AG)CC
HB18	GGCGGCGGCGGCTCCGGTGGTGGTGATGTGAACTTGGAAGTGTC
HB19	GGCGGCGGCGGCTCCGGTGGTGGTGAGGTGAAGGTCATCGAGTC
Variable heavy chain reverse primers
HF1	ATGCGCGGCCGCCGAGGAAACGGTGACCGTGGT
HF2	ATGCGCGGCCGCCGAGGAGACTGTGAGAGTGGT
HF3	ATGCGCGGCCGCCGCAGAGACAGTGACCAGAGT
HF4	ATGCGCGGCCGCCGAGGAGACGGTGACTGAGGT