Generation and Characterization of an scFv Directed against Site II of Rabies Glycoprotein

Table 2

(a) Location of selected epitopes present on PV GP and its size in base pairs (bp). (b) Primers used for amplification of epitope coding regions of PV GP and cloning into pGEX vector.

(a)

Epitope gene ID	Location on PV GP	Size in bp
E1	3318–3640	322
E2	3641–3888	247
E3	3889–4134	238
E4	4135–4380	245
E5	4381–4697	316

(b)

Epitope 1 For	ATCGGGATCCGGAGTATTTTTCAATGGTATAAT
Epitope 1 Rev	ATCGGAATTCTCCTCTGAGATTGTGTTGT
Epitope 2 For	ATCGGGATCCGGAGTATTTTTCAATGGTATAAT
Epitope 2 Rev	ATCGGAATTCCCGTTCGTGCACATCG
Epitope 3 For	ATCGGGATCCAAGAACGGTGACGAGG
Epitope 3 Rev	ATCGGAATTCCCGTTCGTGCACATCG
Epitope 4 For	ATCGGGATCCCAGCAACATATGGAGTTGT
Epitope 4 Rev	ATCGGAATTCCAAGGCAGTCAGGGCC
Epitope 5 For	ATCGGGATCCTGCCCAAACAATTTGGTA
Epitope 5 Rev	ATCGGAATTCCCCGTTCATTTTTATGGC