Effect of Growth Regulators on In Vitro Morphogenic Response of Boscia senegalensis (Pers.) Lam. Poir. Using Mature Zygotic Embryos Explants

Table 1

Effect of different PGRs benzyl adenine (BA), thidiazuron (TDZ), α-naphthalene acetic acid (NAA), and 2, 4-dichlorophenoxy acetic acid (2, 4-D) concentrations on in vitro morphogenesis on B. senegalensis embryo explants after 4 weeks of culture.

PGR mg/L				Number of shoots per explant	Shoot length (cm)	Degree of callus^∗ formation
BA	TDZ	NAA	2, 4-D
0	0	0	0	0.0±0.0^g	0.0	—
1	0	0	0	3.2±0.7^d	2.5 ± 0.5	—
2	0	0	0	10±0.4^c	1.2 ± 0.1	—
3	0	0	0	14.8±0.6^a	0.6 ± 0.0	—
5	0	0	0	10.7±0.8^b	0.4 ± 0.1	—
0	1	0	0	0.0±0.0^g	0.0 ± 0.0	+
0	2	0	0	0.0±0.0^g	0.0	+
0	3	0	0	0.0±0.0^g	0.0 ± 0.0	+
0	5	0	0	0.0±0.0^g	0.0 ± 0.0	+
0	0	1	0	1.0±0.0^e	2.0 ± 0.1	—
0	0	2	0	1.0±0.0^e	1.5 ± 0.1	—
0	0	3	0	0.8±0.2^ef	1.3 ± 0.1	—
0	0	5	0	0.5±0.2^f	0.4 ± 0.2	+
0	0	0	1	0.0±0.0^g	0.0 ± 0.0	+
0	0	0	2	0.0±0.0^g	0.0 ± 0.0	++
0	0	0	3	0.0±0.0^g	0.0 ± 0.0	+++
0	0	0	5	0.0±0.0^g	0.0 ± 0.0	+++

Each value represents the mean + standard error of at least ten replicates. *Degree of callus formation was represented as: —; no callus formed, +; slight callus, ++; moderate callus; +++; massive callus formation.