In Vitro somatic embryogenesis in Dalbergia sissoo. (a) Development of embryogenic callus from immature zygotic embryos on MS medium + 1.0 mg/l Kn, 2.0 mg/l 2,4-D after 4 weeks of culture. (b) Primary and secondary somatic embryogenesis on medium having 1.0 mg/l Kn, 2.0 mg/l 2,4-D after 4 weeks of subculture. (c) Scanning Electron Microscopy (SEM) of globular embryos. (d) Globular somatic embryos. (e) SEM of germination of somatic embryos on strength MS medium supplemented with 0.1 mg/l IAA, 0. 25 mg/l BA and 2% sucrose after 2 weeks of culture. (f) Somatic embryos with cotyledons and radicle.
