Somatic Embryogenesis in Four Tree Legumes

Table 1

Effect of cytokinins and auxins on induction of embryogenic callus from immature zygotic embryos of Dalbergia sissoo (DS), Acacia catechu (AC), Acacia arabica (AA), and Hardwickia binata (HB).

MS + growth regulator (mg/l)				Percent of explant response (Mean±SE)∗
BA	Kn	NAA	2,4-D	DS	AC	AA	HB
0	0.25	0.5	0	20.4±0.6(NE)	18.6±0.7(NE)	31.2±0.6(NE)	29.4±0.6(NE)
0	0.25	1.0	0	24.2±0.8(NE)	28.8±0.6(NE)	36.4±0.7(NE)	32.2±0.8(NE)
0	0.25	2.0	0	54.8±0.7(NE)	42.4±0.7(E)	42.8±0.6(E)	45.6±0.7(NE)
0	0.5	2.0	0	62.4±0.8(NE)	50.6±0.8(E)	46.6±0.8(E)	50.2±0.8(NE)
0	0.5	3.0	0	72.8±0.6(NE)	62.5±0.6(E)	58.8±0.7(E)	60.2±0.6(NE)
0.25	0	1.0	0	26.2±0.7(NE)	30.4±0.9(NE)	28.7±0.9(NE)	34.4±0.9(NE)
0.50	0	2.0	0	34.6±0.8(NE)	44.3±0.6(NE)	46.2±0.7(NE)	52.1±1.0(NE)
1.00	0	3.0	0	54.2±0.7(NE)	61.6±0.8(NE)	56.4±0.6(NE)	56.6±0.4(NE)
0	0.25	0	1.0	32.8±0.9(E)	42.2±0.7(E)	46.9±0.2(E)	52.6±0.8(E)
0	0.25	0	2.0	45.8±0.6(E)	56.7±0.8(E)	62.2±0.5(E)	66.2±0.9(E)
0	0.5	0	3.0	68.8±0.8(E)	60.4±1.0(E)	72.5±0.7(E)	78.4±0.8(E)
0	1.0	0	2.0	75.2±0.6(E)	58.8±0.7(E)	64.4±0.8(E)	66.2±0.7(E)
0	1.5	0	2.0	76.2±0.8(E)	66.2±1.1(E)	72.3±0.7(E)	76.6±1.0(E)
0.25	0	0	2.0	56.6±0.7(NE)	45.6±0.8(NE)	52.5±0.6(NE)	56.9±1.1(NE)
0.50	0	0	3.0	64.4±0.6(NE)	54.2±1.0(NE)	58.2±0.5(NE)	68.4±0.5(NE)e

^∗20 Replicates per culture; repeated thrice. NE—Non-embryogenic Calli, E—Embryogenic Calli.