Somatic Embryogenesis in Four Tree Legumes

Table 2

Development of different sizes of somatic embryos from embryogenic callus of Dalbergia sissoo (DS), Acacia catechu (AC), Acacia arabica (AA), and Hardwickia binata (HB) cultured on different induction medium after 4 weeks of subculture.

Culture medium + 3% (w/v) sucrose	No of somatic embryos per 500 mg embryogenic calli (Mean±SE)∗
	DS	AC	AA	HB
MS + 0.25 mg/l Kn + 1.0 mg/l NAA	52.4 ±0.7	0	0	0
MS + 0.5 mg/l Kn + 1.5 mg/l 2,4-D	24.8 ±0.6	108.2±1.3	125.2±0.8	85.6±0.5
MS + 1.0 mg/l Kn + 2.0 mg/l 2,4-D	20.6±0.7	126.4±1.2	132.6±0.6	122.2±0.8
MS + 1.5 mg/l Kn + 2.0 mg/l 2,4-D	25.7±0.9	134.2±0.8	134.6±0.8	111.6±0.6
MS + 1.5 mg/l Kn + 2.0 mg/l 2,4-D + 400 mg/l L-proline	42.4±1.1	152.6±0.7	168.4±1.2	121.7±1.2
MS + 1.5 mg/l Kn + 2.0 mg/l 2,4-D + 600 mg/l L-proline	46.3±0.6	160.1±1.5	172.6±1.3	128.4±1.1

(10 replicates per treatment; repeated thrice)
∗Data collected after four weeks of culture on proliferation medium.