Research Article
Generation of HIV-1 and Internal Control Transcripts as Standards for an In-House Quantitative Competitive RT-PCR Assay to Determine HIV-1 Viral Load
Table 2
RNA concentration of the HIV-1 and IC transcripts determined by spectrophotometry at 260 nm.
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AU: absorbance units. OD: optical density. A260/280 ratio: the ratio of absorbance at 260 and 280 nm. aTranscript treated with 2 u of RNase-free DNase per 1 μg of DNA used in the in vitro transcription. bTranscript treated with 4 u of RNase free DNase-per 1 μg of DNA used in the in vitro transcription. |