Production of a Thermostable and Alkaline Chitinase by Bacillus thuringiensis subsp. kurstaki Strain HBK-51

Table 2

Influence of chemicals on chitinase activity.

Chemical	Con.	Act. (%)
control		100
DMSO	1%	92
	5%	92
EDTA	1 mM	95
	5 mM	93
Ethyl acetimidate	1 mM	90
	5 mM	80
SDS	1 mM	97
	5 mM	93
Phenol Gliaksol	1 mM	92
	5 mM	104
1,10-Phenanthroline	1 mM	90
	5 mM	100
N-Ethylmaleimide	1 mM	106
	5 mM	89
PMSF	1 mM	108
	5 mM	81
Urea	1 mM	106
	5 mM	80
Na-Sulphite	1 mM	98
	5 mM	96
MgCl2	1 mM	100
	5 mM	90
NaCl2	1 mM	97
	5 mM	93
BaCl2	1 mM	101
	5 mM	87
FeCl3	1 mM	81
	5 mM	106
MnCl2	1 mM	118
	5 mM	77
CuCl2	1 mM	127
	5 mM	155
CoCl2	1 mM	115
	5 mM	116
NiCl2	1 mM	132
	5 mM	123
ZnCl2	1 mM	118
	5 mM	106
KCl2	1 mM	127
	5 mM	144
CaCl2	1 mM	94
	5 mM	106
HgCl2	1 mM	93
	5 mM	89

Con: Concentration, Act: Activity.
The enzyme was incubated with various ions and reagents at room temperature for 30 min., then chitinase activity was assayed under standard conditions. The enzyme activity without any additive was taken as 100%.