Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep

Table 1

Region, methods, primer's sequence (5^'→3^'), and length of PCR products of the ovine myostatin gene.

Region	Using method	Primer's sequence (5^'→3^')	Length of fragment (bp)
Intron 1	PCR-SSCP	F: TAC CTT CAT CAC TCT GCC TTC C	222
		R: GGA GGA AAG AAG AGG GAC AAG
Intron 2	PCR-SSCP	F: CAC ATT TTT CCC CCA GAA GAG	311
		R: AAG ACA GTT CAG AAA ATA GCT GG
Exon 3	PCR-RFLP	F: CCG GAG AGA CTT TGG GCT TGA	337
		R: TCA TGA GCA CCC ACA GCG GTC

F: forward and R: reverse.