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Biotechnology Research International
Table of Contents
Biotechnology Research International
/
2012
/
Article
/
Tab 2
/
Research Article
Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using
PCR-RFLP
and
PCR-SSCP
Techniques in Zel Sheep
Table 2
PCR
conditions.
Location
Primary denaturation in 1st cycle
Denaturation
Annealing
Elongation
Final extension
Number of cycles
°
C/Sec
°
C
Sec
°
C
Sec
°
C
Sec
°
C/Sec
Intron 1
95/240
94
60
56.5
70
72
75
72/600
40
Intron 2
95/240
95
50
55
60
72
75
72/600
40
Exon 3
94/240
94
60
58.5
60
72
120
72/240
35