Polymorphism of Myostatin Gene in Intron 1 and 2 and Exon 3, and Their Associations with Yearling Weight, Using PCR-RFLP and PCR-SSCP Techniques in Zel Sheep

Table 2

PCR conditions.

Location	Primary denaturation in 1st cycle	Denaturation		Annealing		Elongation		Final extension	Number of cycles
	^°C/Sec	^°C	Sec	^°C	Sec	^°C	Sec	^°C/Sec	𝑛
Intron 1	95/240	94	60	56.5	70	72	75	72/600	40
Intron 2	95/240	95	50	55	60	72	75	72/600	40
Exon 3	94/240	94	60	58.5	60	72	120	72/240	35