Recent Advances in the Genetic Transformation of Coffee
Figure 1
Agrobacterium tumefaciens mediated transformation and regeneration of C. canephora cv. C × R and C. arabica genotypes. (b, c, d, f, and k) C. arabica (a, e, g, h, i, j, and l) C. canephora. (a) Cocultivated hypocotyls of in vitro seedlings expressing transient GUS expression; (b) embryogenic calli showing transient GUS expression following cocultivation; (c) initiation of somatic embryos from the transformed calli showing green fluorescence; (d) mass of heart shaped somatic embryo showing green fluorescence; (e) germinating somatic embryo with well-developed cotyledon leaves with bright green fluorescence; (f) Gus expression in the root tips of a germinated transformed plant; (g) strong GFP expression in transgenic root; (h) in vitro plant regeneration; (i) Gus staining of the leaf of a transgenic plant; (j) GFP expression in the developing leaf; (k) GUS staining of the regenerated transformed plant; (l) transgenic plant in the soil.
