Recent Advances in the Genetic Transformation of Coffee

Table 1

Summary of transformation studies in Coffea sp^*.

DNA delivery method	Coffea species	Explants used	Binary vector	Agrobacterium strain	Promoter	Selection marker	Target gene	Results	Reference	Country
Direct delivery electroporation	C. arabica	Protoplast	NA	—	pGA472	nptII	uidA	TE	[15]	USA
	C. arabica	SE	pCAMBIA 3201	—	CaMV35S	bar	uidA	TGI	[16]	Venezuela
	C. canephora	Endosperm	pCAMBIA 1301	—	CaMV35S	hpt	uidA	TE	[17]	India
Microprojectile bombardment	C. arabica	Leaf	pPIGK	—	EF-1a	bar	uidA	TE	[18]	France
	C. arabica	ET	pCAMBIA 2301	—	CaMV35S	nptII	uidA	TGI	[19]	Colombia
	C. arabica	ET	pBI-426	—	CaMV35S	nptII	uidA	TGI, PR	[20]	Brazil
	C. canephora	ET	pCAMBIA 3301	—	CaMV35S	bar	uidA	TGI, PR	[21]	Brazil
	C. arabica	SE	pCAMBIA 2301	—	CaMV35S	nptII	uidA	TE	[22]	Costa Rica
	C. arabica	ET	pBI-426	—	CaMV35S	nptII	uidA	TGI, PR	[23]	Brazil
Indirect delivery A. tumefaciens	C. arabica	Protoplast	pGV2260	NA	CaMV35S	hpt	uidA	TE	[24]	France
	C. canephora	ET	pIGI121-Hm	EHA101		hpt	uidA	TGI, PR	[25]	Japan
	C. arabica and C. canephora	SE	pBIN19	LBA4404	EF-1α	csr-1-1	cry1Ac	TGI, PR	[26]	France
	C. canephora	ZE, SE, H	pBECKS 400	EHA101	CaMV35S	hpt	uidA	TGI,	[27]	India
	C. arabica and C. canephora	ET, SE	pHIBI –IG	EHA101	CaMV35S	hpt	gfp & CaMXMTI	RNAi, PR	[28]	Japan
	C. canephora	H	pBECKS 400	EHA 101	CaMV35S	hpt	uidA	TGI, PR	[29]	India
	C. canephora	ET	pCAMBIA 3301	EHA105	CaMV35S	ppt	uidA	TGI	[30]	Brazil
	C. canephora	ET, Leaf	pER10W-35SRed	C58	CaMV35S	nptII	DsRFP	TGI, PR	[31]	Mexico
	C. canephora	SE	pCAMBIA 1381	A4, EHA 101	CaMV35S	hpt	NMT	RNAi	[32]	India
	C. canephora	SE	pBIN19	LBA 4404	EF-1	csr-1-1	cry1Ac	Field test	[33]	France
	C. arabica	ET	pCAMBIA 3300	EHA 105	CaMV35S	bar	ACC-oxidase	AE	[34]	Brazil
	C. canephora	ET	pCAMBIA 3301	EHA 105	CaMV35S	bar	uidA	TGI, PR	[35]	Brazil
	C. canephora	Embryos	pER10W-35SRed	C58C1	CaMV35S	WUSCHEL	DsRFP	TGI,PR	[36]	Mexico
	C. canephora and C. arabica	ET	pBECKS 2000	EHA 101, EHA 105, LBA 4404, AGL 1	CaMV35S	hpt, visual	uidA, sgfp	TGI, PR	[37]	India
	C. arabica	ET	pMDC32	LBA1119	CaMV35S	hpt	sgfp	PR	[38]	France
A. rhizozenes	C. arabica and C. canephora	SE	pBIN 19	A4	CaMV35S	hpt	uidA	TGI, PR	[39]	France
	C. arabica	Leaf	NA	IFO 14554		NA	NA	TGI, PR	[40]	Japan
	C. canephora	SE	pBIN 19	A4	NA	csr-1-1	cry1Ac	TGI, PR	[41]	France
	C. canephora	SE	pCAMBIA 1301	A4	CaMV35S	hpt	uidA	TGI, PR	[42]	India
	C. arabica	H	pBIN 19	A4	CaMV35S	visual	uidA	TGI, PR	[43]	France
	C. arabica	H	pCAMBIA 2300	A4	CaMV35S	visual	gfp	TGI, PR	[44]	France
					CaMV35S

^*Updated from Etienne et al. [45]; ET: embryogenic tissue; SE: somatic embryos; H: hypocotyls; TE: transient gene expression; TGI: target gene integration; PR: plant rregeneration; RNAi: RNA interference, AE: antisense expression.