Optimization of the Nutritional Parameters for Enhanced Production of B. subtilis SPB1 Biosurfactant in Submerged Culture Using Response Surface Methodology
Table 4
Three variable CCD design with experimental and predicted values of biosurfactant production by Bacillus subtilis SPB1.
Exp N°
Glucose (g/L) ()
K2HPO4 (g/L) ()
Urea (g/L) ()
Biosurfactant yield (g/L)
Experimental value
Predicted value
1
−1 (20)
−1 (1)
−1 (3)
2.900
3.005
2
1 (40)
−1 (1)
−1 (3)
2.400
2.299
3
−1 (20)
1 (2)
−1 (3)
2.600
2.642
4
1 (40)
1 (2)
−1 (3)
2.300
2.337
5
−1 (20)
−1 (1)
+1 (9)
2.800
2.694
6
1 (40)
−1 (1)
+1 (9)
2.100
1.989
7
−1 (20)
1 (2)
+1 (9)
2.400
2.432
8
1 (40)
1 (2)
+1 (9)
2.300
2.127
9
−1.682 (13.18)
0 (1.5)
0 (6)
2.700
2.624
10
+1.682 (46.82)
0 (1.5)
0 (6)
1.600
1.774
11
0 (30)
−1.682 (0.66)
0 (6)
2.900
2.993
12
0 (30)
+1.682 (2.34)
0 (6)
2.800
2.804
13
0 (30)
0 (1.5)
−1.682 (0.95)
2.600
2.518
14
0 (30)
0 (1.5)
+1.682 (11.05)
1.900
2.080
15
0 (30)
0 (1.5)
0 (6)
2.700
2.877
16
0 (30)
0 (1.5)
0 (6)
3.000
2.877
17
0 (30)
0 (1.5)
0 (6)
2.800
2.877
18
0 (30)
0 (1.5)
0 (6)
3.000
2.877
19
0 (30)
0 (1.5)
0 (6)
2.900
2.877
represents the coded level of variables. represent the actual level of variables. Figures in parentheses denote actual level of variables.
