Optimization of the Nutritional Parameters for Enhanced Production of B. subtilis SPB1 Biosurfactant in Submerged Culture Using Response Surface Methodology

Table 6

ANOVA analysis for biosurfactant production in central composite design experiments.

Source of variation	Sum of squares	Degree of freedom	Mean square	𝐹-value	Significance
Regression	2.4734	9	0.2748	10.7150	***
Residual	0.2308	9	0.0256
Lack of fit	0.1628	5	0.0326	1.9157	27.4%
Pure error	0.0680	4	0.0170
Total	2.7042	18