Functional Cloning and Expression of the Schizophyllum commune Glucuronoyl Esterase Gene and Characterization of the Recombinant Enzyme
Figure 6
Separation of substrate and product components in reaction mixtures by (a) TLC: 1a, b (substrate I & product), 2a, b (II & product), 3a, b (III & product), 4a, b (IV & product); (b) HPLC: peak 1, 2 for the acid (product) and ester of substrate V, respectively. Reaction conditions: 20 mM substrate in 50 mM sodium phosphate buffer, pH 6.0, 1 μg rScGE, 30°C, 30 min. See details on TLC and HPLC runs in “Methods” section.
