Family-Specific Degenerate Primer Design: A Tool to Design Consensus Degenerated Oligonucleotides

Figure 2

Primer distribution along one ORF. A collection of the best scoring primers for the nucleoprotein of Arenavirus, comprised of 50 primers for the genomic sequence and 50 for the antigenomic sequence, were represented in the corresponding alignment position. The height of each point indicates the cumulative number of primers corresponding at this position. The alignment was made with 71 arenavirus N protein sequences.