Family-Specific Degenerate Primer Design: A Tool to Design Consensus Degenerated Oligonucleotides

Figure 5

Comparison of FAS-DPD designed primers and minimum degenerated substrings. Collection of primers with the highest score designed for all the ORFs shared by all the genomes used were compared against the minimum degenerated subsequence of the same length for each ORF in order to know how much more degenerated they are. The number below each bar indicates the ratio of degeneration between the designed primer and the minimum degeneration substring. The number above each bar indicates the amount of primers that correspond with the ratio mentioned before. The percentages are cumulative with respect to increasing degeneration ratios and referred to the total number of primers used in the test.