Display of the Viral Epitopes on Lactococcus lactis: A Model for Food Grade Vaccine against EV71
Figure 3
Analysis of serum from immunized mice using VP1 coated ELISA plates. 1: serum of mice immunized with L. lactis displaying VP11-67aa; 2: serum of mice immunized with L. lactis displaying VP11-67aa and VP135-100aa; 3: serum of mice immunized with L. lactis displaying VP135-100aa; 4: serum of control mice immunized with L. lactis; 5: serum of control mice immunized with PBS; 6: serum of the rabbit; 7: serum of preimmunized mice; 8: rabbit anti-VP1 antibodies as positive control. Sera from immunized mice (Balb/c) were taken after 3rd booster immunization. Testing of sera was done at sera dilution 1 : 10,000. Note: the absorbance value shown was after the deduction of the background value obtained from purified His-tag protein.
