Display of the Viral Epitopes on Lactococcus lactis: A Model for Food Grade Vaccine against EV71

Figure 4

Determination of antibody titers by ELISA. (a) Serum from mice immunized with L. lactis displaying VP1_1-67aa, (b) serum from mice immunized with L. lactis displaying VP1_35-100aa, (c) serum from mice immunized with L. lactis displaying both epitopes (VP1_1-67aa and VP1_35-100aa), and (d) serum from control mice immunized with PBS. (e) Serum from control mice immunized with L. lactis. Antibodies were measured using complete VP1 protein coated ELISA plates. Sera from mice (Balb/c) were taken before and after each immunization with L. lactis displaying VP1_1-67aa.

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