Display of the Viral Epitopes on Lactococcus lactis: A Model for Food Grade Vaccine against EV71
Figure 5
Detection of serum antibody response against VP11-67aa and VP135-100aa epitopes of EV71 in mice immunized with L. lactis displaying AcmA/VP135-100aa. Lane 1: BSA protein; lane 2: E. coli (pRSET) total proteins; lane 3: total proteins of L. lactis MG1363; lane 4: purified AcmA/VP11-67aa proteins; lane 5: purified AcmA/VP135-100aa protein; lane M: protein marker (Fermentas, Hanover, MD, USA). The arrow shows recombinant fusion proteins: AcmA/VP11-67aa (~28 kDa) and AcmA/VP135-100aa (~25 kDa).
