Antidepressant Fluoxetine Modulates the In Vitro Inhibitory Activity of Buffalo Brain Cystatin: A Thermodynamic Study Using UV and Fluorescence Techniques

Figure 4

UV-vis spectroscopy of cystatin in the presence and absence of fluoxetine. BC concentrations were fixed at 1 μM while the concentration of fluoxetine was varied from 0.5 μM to 2 μM. Absorption spectra of native BC and in the presence and absence of fluoxetine were recorded in the range of 200–300 nm, cuvette of 1 cm path length for 30 min in the final reaction volume of 1 mL in 0.05 M sodium phosphate buffer pH 7.5.