Research Article
Purification, Characterization of L-Methioninase from Candida tropicalis, and Its Application as an Anticancer
Table 2
Effect of different pH values on the purified L-methioninase activity.
| | pH | Type of buffer | Enzyme activity (U/ml) | Relative activity (%) |
| | 4.0 | A | 128.33 ± 55 | 38.35 | | B | — | — |
| | 5.0 | A | 252.10 ± 60 | 75.35 | | B | — | — |
| | 5.5 | A | 274.94 ± 74 | 82.17 | | B | — | — |
| | 6.0 | A | 302.50 ± 69 | 90.41 | | B | 260.34 ± 80 | 90.92 |
| | 6.5 | A | 334.57 ± 88 | 100 | | B | 286.35 ± 82 | 100 |
| | 7.0 | A | — | — | | B | 260.79 ± 53 | 91.10 |
| | 7.5 | A | — | — | | B | 245.35 ± 82 | 85.68 |
| | 8.0 | A | — |
— | | B | 228.0 ± 75 | 79.63 |
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A: sodium citrate buffer; B: potassium phosphate buffer.
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