Research Article
Response Surface Optimization of Bioethanol Production from Sugarcane Molasses by Pichia veronae Strain HSC-22
Table 2
Experimental design matrix with experimental and predicted bioethanol yield.
| Run number |
Initial pH
|
Molasses concentration
|
Incubation temperature
|
Mixing rate
|
Incubation period
|
Bioethanol concentration g/L | Bioethanol yield | % | Actual | Predicted |
| 1 | 0 | −1 | 0 | 0 | 0 | 18.2 | 41.4 | 39.1 | 2 | −1 | −1 | −1 | −1 | +1 | 4.01 | 9.13 | 11.2 | 3 | 1 | −1 | −1 | −1 | +1 | 6.29 | 14.3 | 13.2 | 4 | 0 | 0 | 0 | −1 | 0 | 19.3 | 33.0 | 34.1 | 5 | 0 | 0 | 0 | 0 | +1 | 18.4 | 31.4 | 29.7 | 6 | −1 | 0 | 0 | 0 | 0 | 15.3 | 26.1 | 22.6 | 7 | 0 | 0 | −1 | 0 | 0 | 20.3 | 34.7 | 34.8 | 8 | +1 | −1 | −1 | +1 | −1 | 4.61 | 10.5 | 13.9 | 9 | +1 | +1 | +1 | +1 | +1 | 22.3 | 30.5 | 26.5 | 10 | −1 | +1 | +1 | −1 | +1 | 19.5 | 26.7 | 23.9 | 11 | −1 | −1 | +1 | −1 | −1 | 3.96 | 9.02 | 10.9 | 12 | −1 | −1 | +1 | −1 | +1 | 6.48 | 14.8 | 16.5 | 13 | +1 | −1 | +1 | −1 | −1 | 4.64 | 10.6 | 10.3 | 14 | 0 | +1 | 0 | 0 | 0 | 23.5 | 32.1 | 38.9 | 15 | +1 | −1 | +1 | +1 | +1 | 8.90 | 20.3 | 24.2 | 16 | 0 | 0 | 0 | 0 | 0 | 20.6 | 35.2 | 35.4 | 17 | 0 | 0 | 0 | +1 | 0 | 22.1 | 37.7 | 37.7 | 18 | −1 | −1 | −1 | +1 | −1 | 4.31 | 9.82 | 10.2 | 19 | 0 | 0 | 0 | 0 | 0 | 21.3 | 36.4 | 35.4 | 20 | −1 | +1 | +1 | +1 | −1 | 8.80 | 12.0 | 13.5 | 21 | 0 | 0 | +1 | 0 | 0 | 21.5 | 36.7 | 37.7 | 22 | +1 | +1 | +1 | +1 | −1 | 9.44 | 12.9 | 13.5 | 23 | +1 | −1 | +1 | −1 | +1 | 7.96 | 18.1 | 15.9 | 24 | 0 | 0 | 0 | 0 | 0 | 21.4 | 36.6 | 35.4 | 25 | +1 | 0 | 0 | 0 | 0 | 11.1 | 19.0 | 23.6 | 26 | 0 | 0 | 0 | 0 | 0 | 20.9 | 35.6 | 35.4 | 27 | 0 | 0 | 0 | 0 | 0 | 21.3 | 36.4 | 35.4 | 28 | +1 | +1 | −1 | +1 | −1 | 11.9 | 16.2 | 13.0 | 29 | −1 | +1 | +1 | +1 | +1 | 19.4 | 26.5 | 26.6 | 30 | 0 | 0 | 0 | 0 | 0 | 21.3 | 36.4 | 35.4 | 31 | −1 | +1 | −1 | −1 | −1 | 8.45 | 11.6 | 11.4 | 32 | +1 | +1 | +1 | −1 | +1 | 14.7 | 20.1 | 22.2 | 33 | +1 | +1 | −1 | −1 | −1 | 10.8 | 14.8 | 12.5 | 34 | −1 | −1 | −1 | −1 | −1 | 3.39 | 7.72 | 7.13 | 35 | +1 | +1 | −1 | +1 | +1 | 17.1 | 23.4 | 24.5 | 36 | 0 | 0 | 0 | 0 | 0 | 20.9 | 35.7 | 35.4 | 37 | +1 | −1 | −1 | −1 | −1 | 3.60 | 8.20 | 9.26 | 38 | +1 | −1 | −1 | +1 | +1 | 10.7 | 24.4 | 22.9 | 39 | 0 | 0 | 0 | 0 | −1 | 10.8 | 18.4 | 21.1 | 40 | 0 | 0 | 0 | 0 | 0 | 20.6 | 35.3 | 35.4 | 41 | +1 | +1 | +1 | −1 | −1 | 10.6 | 14.5 | 14.2 | 42 | −1 | −1 | +1 | +1 | +1 | 9.54 | 21.7 | 23.4 | 43 | +1 | −1 | +1 | +1 | −1 | 7.07 | 16.1 | 13.7 | 44 | +1 | +1 | −1 | −1 | +1 | 13.6 | 18.5 | 19.0 | 45 | 0 | −1 | +1 | +1 | −1 | 6.26 | 14.3 | 12.7 | 46 | 0 | +1 | −1 | −1 | +1 | 12.9 | 17.7 | 17.9 | 47 | 0 | +1 | −1 | +1 | −1 | 7.31 | 9.99 | 10.3 | 48 | 0 | +1 | −1 | +1 | +1 | 15.7 | 21.5 | 21.9 | 49 | 0 | +1 | +1 | −1 | −1 | 12.2 | 16.7 | 15.8 | 50 | 0 | −1 | −1 | +1 | +1 | 8.74 | 19.9 | 19.3 |
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