The Extent of Ventilator-Induced Lung Injury in Mice Partly Depends on Duration of Mechanical Ventilation

Figure 6

Inflammatory mediators. ((a)-(b)) Protein levels of the proinflammatory cytokines interleukin (IL)-1β and IL-6 were determined in supernatant of total lung homogenates (HMG). ((c)–(d)) In addition, protein levels of the chemotactic cytokines keratinocyte-derived chemokine (KC) and macrophage inflammatory protein (MIP)-2 were determined. Data are presented as scatter plot (median) of 7-8 mice per group (triangle = NVC; circle = LV_T; square = HV_T). *Illustrates primary statistical analysis (*𝑃<0.05, **𝑃<0.01); ^#illustrates secondary statistical analysis (^#𝑃<0.05, ^##𝑃<0.01). NVC = nonventilated controls; LV_T, HV_T = LV_T/PEEP or HV_T/ZEEP ventilator settings; 5 h, 12 h = 5 or 12 hours of ventilation.

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