]>Comparative Study of Regulatory T Cell Function of Human <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msup><mml:mtext>CD25</mml:mtext><mml:mtext>+</mml:mtext></mml:msup></mml:math><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msup><mml:mtext>CD4</mml:mtext><mml:mtext>+</mml:mtext></mml:msup></mml:math> T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood : Figure 2
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305859.fig.002b
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Figure 2: Lack of suppressive activity and absence of an anergic state by CB CD25+CD4+ T cells. CD4+CD25+ and CD4+CD25− T cells were purified by a magnetic microbead system. (a) Cells were lysed and immunoblotted with anti-FOXP3 Ab (top panel) or anti-β-actin Ab (bottom panel). (b) Cells were stimulated with anti-CD3/CD28-mAb-coated beads under the indicated conditions. Cell proliferation was assessed by 3H-thymidine uptake on day 4. Data are representative of 5 different donors.