| Figure 6: Restoration of suppressive activity in
expanded CB CD25+CD4+ T cells. (a) Freshly
isolated CD4+CD25+ cells were used as Treg in
Figure 2(b), and expanded as described in
Section 2. Freshly isolated CD25−CD4+ T cells were used
as responder cells in both primary and secondary
assays without expansion. Cell proliferation was assessed by 3H-thymidine
uptake on day 4. Data are obtained by
experiments with samples of 3
different donors. (b) Freshly isolated
CD4+CD25+ cells or expanded CD4+CD25+ cells
were stained with anti-CD3, CD25, and CD45RA, and analyzed using
FACSCalibur. The cells were gated for
CD3+CD25+ cells and the expression of CD45RA was compared
between freshly isolated CD4+CD25+ cells (open histogram)
and expanded CD4+CD25+ cells (closed histogram).
Data are representative of 3 different donors. (c) Expression of FOXP3 protein
was compared between freshly isolated cells and expanded cells by Western
blot. Data are representative of 3
different donors. |
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