Valsartan Attenuated Homocysteine-Induced Impaired Autophagy and ER Stress in Human Umbilical Vein Endothelial Cells

Figure 4

Knockdown of AT1 receptor prevented Hcy-induced impaired autophagy, ER stress, and apoptosis in HUVECs. (a) Representative immunoblots analysis of AT1 protein in HUVECs transfected with AT1 siRNA. (b) Cell viability was assessed by CCK8 assay in Hcy-treat HUVEC cells with AT1 knockdown. (c, d) Representative immunoblots and corresponding densitometry analysis of LC3B, Beclin1, and p62 protein in Hcy-treated HUVECs with or without AT1-siRNA. (e, f) Representative immunoblots and corresponding densitometry analysis of BiP, IRE1α, XBP1, ATF4, and CHOP protein in Hcy-treated HUVECs with or without AT1-siRNA. (g, h) Representative immunoblots and corresponding densitometry analysis of Bax, Bcl2, and cleaved-caspase 3 protein in Hcy-treated HUVECs with or without AT1-siRNA. Data are shown as ; compared with si-NC group; ^# compared with Hcy+si-NC group.

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