Figure 2: Respiratory growth of S. cerevisiae mutant cells expressing M. tuberculosis mtFabD. Wild-type yeast cells harboring a YEplac195 plasmid vector and cells similarly transformed to uracil prototrophy or expressing mitochondrially targeted Rv2243 (mtFabD) from a YEp352 plasmid (both plasmid types are marked with URA3) were grown on SD-Ura medium that selected for plasmid presence, and following serial dilution (triangle), were applied to solid media that consisted of (a) 3% (wt/vol) glycerol (SCglycerol) or (b) 2% (wt/vol) glucose (SD-Ura). The plates were incubated at until single colonies appeared, and recorded photographically. For (c), following an additional 4 d incubation, the SD-Ura plate was overlaid with 0.1% (wt/vol) 2,4,5-triphenyltetrazolium chloride (TTC), and the development of the red chromophore was monitored.
