Antibiotic Combinations with Daptomycin for Treatment of Staphylococcus aureus Infections

Table 3

In vitro synergy of antibiotic combinations.

Method	Strain	Combination	Observation	Ref.
Etest and time-kill study	hGISA/MSSA	Daptomycin with vancomycin, gentamicin, rifampin, linezolid, quinupristin/ dalfopristin, ampicilin-sulbactam	Etest: additive effect with daptomycin + vancomycin and daptomycin + gentamycin, other combinations with daptomycin indifferent Time-kill study: additive effect with daptomycin + gentamycin, other combinations with daptomycin indifferent	[40]
Etest and time-kill study	GISA/MRSA	Daptomycin with vancomycin, gentamicin, rifampin, linezolid, quinopristine/ dalfopristine, ampicilin-sulbactam	Etest: additive effect only with daptomycin + gentamycin, other combinations with daptomycin indifferent Time-kill study: indifference	[40]
Time-kill study	MRSA	Daptomycin + gentamicin	Synergy in all three strains	[41]
Time-kill study	MRSA	Daptomycin + rifampin	Antagonism in one strain and indifference in two other strains	[41]
Time-kill study	MRSA	Daptomycin at 0.0625 MIC to 2 MIC + oxacillin or ampicillin- sulbactam	Synergy of daptomycin at 0.5 MIC + oxacillin 32 mg/L or ampicillin-sulbactam 2–8 mg/L (ampicillin)	[57]
In vitro model of biofilm	MSSA	Daptomycin 10 mg/kg/d + clarithromycin 250 mg q12 h	Sustained bactericidal activity against planctonic and biofilm bacteria	[54]
In vitro model of biofilm	MRSA	Daptomycin 10 mg/kg/d + rifampin at 600 mg q24 h	Sustained bactericidal activity against planctonic and biofilm bacteria	[54]
In vitro selection for resistance	MRSA and GISA	Daptomycin + 0.25 MIC ampicillin, amoxicillin-clavulanic acid, gentamicin, rifampin	Combination with ampicillin, amoxicillin-clavulanic acid delayed/prevented occurrence of non-susceptibility; rifampin delayed non-susceptibility	[58]
Checkerboard method	MRSA, MSSA	Daptomycin + rifampicin, moxifloxacin or fusidic acid	Daptomycin + fusidic acid: antagonism in one MSSA strain reported	[68]

MRSA: meticillin-resistant S. aureus; MSSA: meticillin-susceptible S. aureus; GISA: glycopeptide-intermediate S. aureus; hGISA: heteroresistant glycopeptide-intermediate S. aureus; MIC: minimal inhibitory concentration (mg/L).