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Figure 3: Localization of fluorescent protein-tagged SR-BI containing or lacking the C-terminal tail in transfected HepG2 cells. HepG2 cells transfected with eGFP-SR-BI ((a)–(c)), eGFP-SR-BI-ΔC ((d)–(f)), mRFP-SR-BI ((g)–(i)), or mRFP-SR-BI-ΔC ((j)–(l)) were fixed, stained with DAPI and imaged by wide field fluorescence microscopy in the blue ((a), (d), (g), and (j)), green ((b) and (e)), or red ((h) and (k)) channels. Merged images of the blue and green ((c) and (f)) or the blue and red ((i) and (l)) channels are also shown. All images are set to the same scale. Scale bar in (a) = 10 μm.