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Cholesterol
Volume 2012 (2012), Article ID 610741, 8 pages
http://dx.doi.org/10.1155/2012/610741
Research Article

In Vivo Inflammation Does Not Impair ABCA1-Mediated Cholesterol Efflux Capacity of HDL

1Department of Vascular Medicine, Academic Medical Center Amsterdam, 1105 AZ Amsterdam, The Netherlands
2Department of Experimental Vascular Medicine, Academic Medical Center Amsterdam, 1105 AZ Amsterdam, The Netherlands
3Department of Gastroenterology, Tytgat Institute for Liver and Intestinal Research, Academic Medical Center Amsterdam, 1105 AZ Amsterdam, The Netherlands

Received 26 October 2011; Revised 13 January 2012; Accepted 27 January 2012

Academic Editor: Andrei C. Sposito

Copyright © 2012 Remco Franssen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

HDL provides atheroprotection by facilitating cholesterol efflex from lipid-laden macrophages in the vessel wall. In vitro studies have suggested impaired efflux capacity of HDL following inflammatory changes. We assessed the impact of acute severe sepsis and mild chronic inflammatory disease on the efflux capacity of HDL. We hypothesize that a more severe inflammatory state leads to stronger impaired cholesterol efflux capacity. Using lipid-laden THP1 cells and fibroblasts we were able to show that efflux capacity of HDL from both patients with severe sepsis or with Crohn's disease (active or in remission), either isolated using density gradient ultracentrifugation or using apoB precipitation, was not impaired. Yet plasma levels of HDL cholesterol and apoA-I were markedly lower in patients with sepsis. Based on the current observations we conclude that inflammatory disease does not interfere with the capacity of HDL to mediate cholesterol efflux. Our findings do not lend support to the biological relevance of HDL function changes in vitro.