610741.fig.003a
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610741.fig.003b
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Figure 3: Efflux potential of HDL isolated from patients with Crohn’s disease in fibroblasts and THP1 cells. Cholesterol acceptor capacity of UC-HDL (black bars) and PEG HDL (white bars) isolated from controls, and patients with active Crohn’s disease and Crohn’s disease in remission using cholesterol-laden fibroblasts and cholesterol-laden THP1 cells. Cells are labelled for 24 h with 3H-cholesterol. Fibroblasts were incubated in the presence of LXR agonist TO901317. Efflux to HDL (10 μg/mL apoA-I) is measured during a 4 h time span. Efflux medium without HDL is used as a negative control. Fractional cholesterol efflux is determined by measuring the release of radio-labeled cholesterol into the medium. The percentage efflux equals the counts in medium divided by total counts in medium and cells combined and was corrected for counts to medium without acceptor HDL. Experiments were performed in triplicate. Data are presented as mean ± SD; .