The Influence of an Obesogenic Diet on Oxysterol Metabolism in C57BL/6J Mice

Table 4

Post-diet oxysterols in plasma, liver tissue, and adipose tissue.

Variables	Plasma (ng/mL)		Liver tissue (ng/mg protein)		Adipose tissue (ng/mg protein)
	ND	HFC	ND	HFC	ND	HFC
4β-OH	6.8±0.7	67.0±9.6^‡	7.2±0.9	18.3±7.7^∗	17.4±8.2	130.9±62.2^†
5,6β-epoxy	3.8±0.4	10.0±3.0^∗	12.3±5.3	11.1±3.2	49.7±36.4	81.8±50.2
5,6α-epoxy	2.8±0.6	10.0±4.1^∗	7.8±2.7	9.2±2.4	33.7±21.1	80.2±67.5
7-keto	1.2±0.3	1.9±0.3	7.2±3.5	7.2±3.0	62.7±41.5	74.6±46.5
7α-OH	1.2±0.1	3.2±1.2^∗	5.4±2.0	4.4±1.5	36.9±21.7	34.8±11.6
7β-OH	1.0±0.1	2.4±0.5^∗	3.2±1.1	3.8±1.3	35.1±25.6	28.3±9.2
25-OH	0.6±0.4	0.7±0.1	5.8±7.4	18.5±30.6	146.5±107.7	52.1±57.1
27-OH	0.7±0.1	2.6±0.3^‡	1.3±0.4	1.9±0.6	18.3±7.5	45.8±16.1^†

Note. Data are presented as mean ± SD. ^∗𝑃<0.05, ^†𝑃<0.01, ^‡𝑃<0.001 significantly different than control group. 𝑛=6 per group. 5,6α-epoxy: 5α,6α-epoxycholesterol; 5,6β-epoxy: 5β,6β-epoxycholesterol; 4β-OH: 4β-hydroxycholesterol; 25-OH: 25-hydroxycholesterol; 27-OH: 27-hydroxycholesterol; 7α-OH: 7α-hydroxycholesterol; 7β-OH: 7β-hydroxycholesterol; 7-keto: 7-ketocholesterol.