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Figure 5: Evaluation of the three extraction methods (auto, semiauto, manual) of KNEWRiTE using four types of artificial 3D neuron images generated by connection of cylinders. (a) Raw: neuron without other objects or noise. (b) Biased background: a large object covers the neuron. (c) Noise: addition of white noise. (d) Object: neuron and overlapping cylindrical object. (e) Mutual consistency: missing existing branches and detecting nonexistent branches. (f) Discrepancy of diameters: discrepancy of diameters between neuron and extracted model. (g) Error of simulation: difference between neuron and extracted model in passive model simulation. (h) Extraction time: time required for extraction. Automatic extraction, which took less than 1 min for each case using conventional PC hardware, is not shown in this graph. The Tukey multiple-comparison test examined differences among groups.