Mimosine As Well As Serum Starvation Can Be Used for Cell Cycle Synchronization of Sheep Granulosa Cells
Figure 1
(a) Normally growing and (b) confluent sheep granulosa cells. The scale bars are 200 μm. (c) DNA synthesis of sheep granulosa cells as assayed by BrdU incorporation. Cells were normally growing. The scale bar is 100 μm. (d) TUNEL assay of sheep granulosa cells grown with 72-hour serum starvation. Arrows indicate TUNEL positive cells (apoptotic cells) that stained very dark. The scale bar is 100 μm.
