Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages

Figure 6

Expression of PCNA in the cells and spheres by H&E staining. Brown nuclei indicated positive PCNA staining. (a) Monolayer SGC9701 cells showed positive PCNA expression. (b, c) The microencapsulated cell spheres cultured for 7 days and 14 days: PCNA expression was observed throughout the entire spheres. (d) The microencapsulated cell spheres cultured for 21 days: PCNA expression was detected outside the spheres, but not in the center. (e) The microencapsulated cell spheres cocultured with macrophages for 3 days: the number and density of the spheres expressing PCNA were increased. Magnification: 200x.

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