Establishment of a Model of Microencapsulated SGC7901 Human Gastric Carcinoma Cells Cocultured with Tumor-Associated Macrophages

Figure 7

Expression of VEGF in the cells and spheres by H&E staining. Brown nuclei indicated positive VEGF staining. (a) Monolayer SGC9701 cells showed positive VEGF expression. (b, c, d) The microencapsulated cell spheres cultured for 7, 14, and 21 days: VEGF expression was observed throughout the entire spheres. (e) The microencapsulated cell spheres cocultured with macrophages for 3 days: the number and density of the spheres expressing VEGF were increased. Magnification: 200x.

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