Hsa_circ_0006988 Promotes Sorafenib Resistance of Hepatocellular Carcinoma by Modulating IGF1 Using miR-15a-5p

Figure 3

Hsa_circ_0006988 serves as a miR-15a-5p sponge. (a) Predicted docking sites for hsa_circ_0006988 and miR-15a-5p. (b) Direct binding between hsa_circ_0006988 and miR-15a-5p, as evidenced by the DLR assay. (c) RIP data confirming the hsa_circ_0006988 interaction with miR-15a-5p. (d) MiR-15a-5p levels, via RT-qPCR, in HepG2, Huh7, SR-HepG2, and SR-Huh7 cells. (e) MiR-15a-5p levels, via RT-qPCR, in sorafenib-resistant (SOR-R) cells incorporated with si-NC or si-hsa_circ_0006988. (f) MiR-15a-5p levels, via RT-qPCR, in sensitive and resistant tissues. (g) Association between hsa_circ_0006988 and miR-15a-5p, as evidenced by Spearman’s correlation coefficient. (h) MiR-15a-5p levels, via RT-qPCR, in SOR-R cells incorporated with NC, or miR-15a-5p inhibitor (miR-I). (i) IC50s of SOR. The presented data are the mean of 3 replicates, and .

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