Hsa_circ_0006988 Promotes Sorafenib Resistance of Hepatocellular Carcinoma by Modulating IGF1 Using miR-15a-5p

Figure 4

MiR-15a-5p targets IGF1 to inhibit chemoresistance in sorafenib-resistant (SOR-R) HCC cells. (a) Predicted docking sites for IGF1 and miR-15a-5p. (b) IGF1 and miR-15a-5p interaction validation, via DLR assay. IGF1 levels, via RT-qPCR (c) and ELISA assay (d). IGF1 levels via RT-qPCR (e) and ELISA assay (f), in HepG2, Huh7, SR-HepG2, and SR-Huh7 cells. (g) IGF1 levels, via RT-qPCR, in sensitive and resistant tissues. (h) IGF1 and miR-15a-5p association, via Spearman’s correlation coefficient. IGF1 levels, via RT-qPCR (i) and ELISA assay (j). (k) IC50s of SOR. IGF1 levels, via RT-qPCR (l) and ELISA assay (m). (n) Hsa_circ_0006988 and IGF1 association, via Spearman’s correlation coefficient. The presented data are mean of 3 replicates, and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)