Immunohistochemical Differentiation between Western and East Asian Types of CagA-Positive Helicobacter pylori in Gastric Biopsy Samples

Figure 1

Specificity of the EPIYA-C and EPIYA-D mAbs by ELISA and Western blotting. The specificity of the novel mAbs or the CagA mAb was examined by ELISA (a) and Western blotting (b) using the sonicated whole cell lysate of a Western type H. pylori (ATCC 43504: lane 1), an East Asian type H. pylori (HpLN-4: lane 2), or an isogenic cagA-knockout H. pylori mutant (ATCC 43504 ΔcagA: lane 3). Among the 3 strains of H. pylori, the EPIYA-C mAb was specific to the Western type and the EPIYA-C mAb was specific to the East Asian type, while the CagA mAb reacted with both the Western and East Asian type CagA protein detected by the novel EPIYA-C or EPIYA-D mAb. Error bars indicate standard deviation of 3 identical wells. (M) Molecular size marker (kDa).

(a)

(b)