Pumilio RNA-Binding Family Member 1 Plays a Promoting Role on Pancreatic Cancer Angiogenesis

Figure 6

Effect of pancreatic cancer cells overexpressed or silenced PUM1 on angiogenesis-related signaling of HUVECs. To construct a transwell co-culture system, MIA PaCa-2 and PANC-1 cells of ov-NC, ov-PUM1, sh-NC, and sh-PUM1 were cultured in the upper chamber of a transwell insert, and HUVECs were cultured in the lower chamber. Protein levels in HUVECs were detected by Western blot.

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