Gambogic Acid Inhibits Gastric Cancer Cell Proliferation through Necroptosis

Figure 2

Nec-1 treatment partially blocked GA-induced cell death. Cells were treated with GA and Nec-1 for 24 hours. Annexin V/PI staining was used to stain the cells to identify apoptotic and necroptotic cells. Annexin V-FITC⁺/PI⁺ cells were considered apoptotic/necroptotic cells at the late stage. (a) AGS cells. (b) HGC27 cells. At least 3 randomly selected fields under the microscope were counted for each sample.

(a)

(b)